Evaluation of 9 Rapid Agglutination Tests for the Diagnosis of Typhoid and Parathyphoid Fever in Côte D'ivoire
André Inwoley1, 2, *, Roseline Affi-Aboli1, Matthieu Kabran1, 2, Aimé Césaire Adiko1, Nadège-Audrey Abiyou1, Franck Stéphane Amani1, Jean-Claude Seka1, Bamory Dembele1
Identifiers and Pagination:Year: 2016
First Page: 243
Last Page: 248
Publisher ID: MEDJ-3-243
Article History:Received Date: 01/09/2016
Revision Received Date: 24/09/2016
Acceptance Date: 24/09/2016
Electronic publication date: 31/10/2016
Collection year: 2016
open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution-Non-Commercial 4.0 International Public License (CC BY-NC 4.0) (https://creativecommons.org/licenses/by-nc/4.0/legalcode), which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
In Africa, the Widal test is done in the majority of laboratories by rapid tests whose performances are poorly documented. We evaluate the performance of 9 commercial rapid tests used in Côte d'Ivoire for the serodiagnosis of typhoid and paratyphoid fever.
we used a panel of 170 samples collected from subjects suspected of typhoid or paratyphoid fever in Abidjan to determine the sensitivity, the specificity and operational characteristics of 9 plate agglutination tests (BIOREX®, BIOTEC®, CHRONOLAB®, CROMATEST®, FORTRESS®, PLASMATEC®, CYPRESS®, SPINREACT® and TYDAL®) compared to the reference technique SALMONELLA SEROLOGY® of BIORAD (tube agglutination).
Among the 9 evaluated tests, only BIOTEC® presented good performances (96% sensitivity and 97% specificity). Others gave sensitivities below 80%. Their characteristics were improved with the semi-quantitative plate agglutination test. All tests had good operational characteristics.
In our context, the majority of tests evaluated should not be used to carry out the Widal test.